Quantitative analysis of proteins – targeted proteomics LC-MS/MS
Quantitative analysis of proteins by LC-MS/MS is a modern research tool used in biotechnology, pharmaceutical industry and clinical diagnostics. Their quantification provides key information about the state of the organism and supports drug design and biomarker research. Thanks to advanced mass spectrometry techniques such as MRM (Multiple Reaction Monitoring) and MRM HR (High-Resolution MRM), precise, sensitive and reproducible determination of specific proteins is possible even at very low concentrations. Compared to traditional methods (e.g., Western blot or ELISA), targeted proteomics does not require the use of antibodies and allows simultaneous detection of multiple proteins in a single analysis.Precision quantitative LC-MS/MS proteomics is ideal for creating extensive panels of biomarkers and studying their changes in large sets of biological samples (e.g., plasma, serum, cell cultures).
Our laboratory uses advanced LC-MS/MS technologies of the latest generation, based on SCIEX ‘s high-resolution (HR) and low-resolution (LR) mass spectrometers – ZenoTOF 7600, QTRAP 5500+ and QTRAP 6500+, which guarantees exceptional sensitivity and accuracy of analyses. LC-MS/MS kits enable precise detection of proteins, which translates into efficient determination of biomarkers present in ultra-low concentrations. Thanks to the variety of instruments, we select the optimal analytical conditions for the specific matrix under study and the purpose of the project.
Technologies and analytical methods used:
- MRM / MRM-HR – precise and selective detection of selected proteins by monitoring characteristic peptide fragments.
- microLC-MS/MS with Optiflow ion source – microfluidic liquid chromatography to increase ionization efficiency and sensitivity.
- ZenoTOF 7600 SCIEX high-resolution mass spectrometer ideal for proteomic analysis and biomarker determination.
- QTRAP 5500+ / 6500+ – targeted proteomics and quantitative analysis of proteins in complex matrices.
- LC-MS/MS data analysis software – SciexOS, PEAKS Studio, Spectronaut, Skyline, Biologics Explorer.
Scope of analytical services
We offer comprehensive quantitative analysis of proteins in a variety of biological matrices and test systems:
- Intact mass analysis – for <20 kDa proteins without digestion.
- Bottom-up proteomics – for proteins >20 kDa, analysis after enzymatic digestion.
- Composite samples (plasma, serum, cell cultures): purification, digestion, method optimization and validation.
Strategies for quantitative proteomic analysis:
- Protein quantification using a calibration curve and internal standard – guarantees high accuracy and repeatability.
- TMT / iTRAQ (isobaric tracers) – multichannel benchmarking.
- Label-free quantification (LFQ) – quantitative analysis without labeling, ideal for large sample sets.
Results of quantitative protein analysis
We provide a complete proteomics report that includes:
- Identification of proteins based on MS/MS spectra.
- Quantitative protein results according to methodology: calibration curve, TMT/iTRAQ, label-free.
- Analysis of protein contaminants – optional.
For whom?
- Scientists and research and development (R&D) units.
- Diagnostic laboratories – supporting the development of protein tests.
- Biotechnology and pharmaceutical companies – quantification of monoclonal antibodies, PK/PD testing.
- Quality control of biological products – detection of impurities, analysis of protein purity.
- Research projects – comparative analysis of multiple samples by LC-MS/MS.
Contact us
Write or call – we offer comprehensive support: method selection, sample preparation, LC-MS/MS measurement, result reporting. Professional LC-MS/MS quantitative protein analysis with guaranteed precision and individual approach.
See also: PQ500, Protein identification, mAB analysis
