Identification of lipids by LC-HR-MS/MS with EAD and CID fragmentation
We provide professional shotgun lipidomics analysis services using advanced LC-HR-MS/MS (Liquid Chromatography-High-Resolution Tandem Mass Spectrometry) technology. Using SCIEX‘s ZenoTOF 7600 high-resolution mass spectrometer for analysis, we offer the ability to use both classical CID fragmentation and state-of-the-art EAD fragmentation for maximum precision identification and characterization of lipid structures. Our services are aimed at scientists, research teams and specialists in molecular biology, analytical chemistry, biotechnology and medicine who need data of the highest quality.
⚡ CID and EAD fragmentation
🔸 CID (Collision-Induced Dissociation) – the classic standard.
- Effective for routine identification of lipid classes
- Excellent for quantitative lipid analysis and rapid profiling
- Reliable for analyzing large numbers of biological samples
🔸 EAD (Electron-Activated Dissociation) – rich fragmentation
- Accurate localization of double bonds and differentiation of isomers
- Analysis of atypical molecular structures, maintaining full fragmentation integrity
- Advanced identification of signal lipids, positional and geometric isomers
With the ability to use both fragmentation methods – CID and EAD – we tailor an analysis strategy to your research goals: from rapid profiling to in-depth structural analysis.
EAD fragmentation of lipids.
✅ Scope of service:
- Identification and profiling of lipids (phospholipids, triacylglycerols, sphingolipids, sterols, ceramides, DAG, MAG and others)
- Quantitative relative and absolute analysis
- Analysis of various biological matrices (cells, tissues, plasma, serum, body fluids, others)
- Rapid LC separation coupled to HR-MS/MS
- Fragmentation option: CID and/or EAD – choice dependent on structural needs and scope of study
- Optimized extraction protocols (Folch, Bligh-Dyer, MTBE)
- Comprehensive reports
📦 What you get:
- Full list of identified lipids with MS/MS annotations (EAD and/or CID)
- Quantitative statements (relative and absolute)
- Raw data (.wiff), processed data (.csv, .xlsx)
- Statistical analyses (on request): PCA, PLS-DA, heatmaps, clustering
- Publishable charts and visualizations
- Scientific consultation and support for publication of results
🧪 Applications of lipidomic analysis:
- Studies of lipid biomarkers in metabolic, neurological and cancer diseases
- Intergroup comparisons (control vs. pathology, diet vs. intervention, etc)
- Assessing the impact of drugs, diet or environmental factors
- Cellular and tissue lipidomics – in vitro and in vivo models
- Pharmacology, toxicology, nutrigenomics, immunology, other
🤝 Why is it worth it?
- Team of experts with expertise in lipidomics, mass spectrometry and bioinformatics
- State-of-the-art LC-HR-MS/MS equipment with EAD and CID options
- Proven software for processing lipidomic data – MS-DIAL, MarkerView, SciexOS
- Flexibility of methods – from rapid screening to in-depth molecular characterization
- Substantive support and assistance in integrating results into grant projects or publications
📩 Contact us
Need precision lipid analysis? We provide accuracy, flexibility and expert support. Contact us for a quote, turnaround time and free methodological consultation.
