Analysis of anabolic steroids

Targeted qualitative analysis of 28 anabolic steroids in urine by LC-HR-MS/MS technique – a next-generation precision laboratory test

Service description

Our laboratory offers highly specialized qualitative analysis of 28 anabolic steroids in urine using LC-HR-MS/MS (high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry) technology. This is one of the most accurate and sensitive methods available for the detection of steroidal compounds – allowing for reliable, selective and rapid detection of the presence of anabolic-androgenic steroids (AAS) and their metabolites in a biological sample.

The test for the presence of anabolic steroids is used in doping analysis, anti-doping controls, sports medicine, clinical research, monitoring of hormonal therapies, and forensic and environmental toxicology, among others. If you want to know if and which anabolic steroids are present in samples – take advantage of the offer carried out by us together with Lab4Tox. Our offer includes highly specific and sensitive qualitative analysis of a wide profile of anabolics by LC-HR-MS/MS technique. The use of high-resolution mass spectrometry (HR-MS and HR-MS/MS) in the analysis of anabolic steroids makes it possible to unequivocally confirm or exclude the presence of a given compound.

Why is it important to analyze 28 anabolic steroids by LC-HR-MS/MS?

• ✅ High sensitivity and selectivity – ability to detect compounds as low as 0.01 to 5 ng/ml depending on the substance

• ✅ Identification of endogenous and exogenous forms of steroids

• ✅ No need for screening analysis – one-step direct targeted qualitative test

• ✅ Detection of a wide spectrum of anabolic substances and their metabolites

Technology and conduct of the study

The analysis is carried out using state-of-the-art LC-HR-MS/MS instruments (SCIEX ZenoTOF 7600), providing exceptional accuracy in measuring the molecular weights, isotopic distribution of the substance and fragmentation spectrum of the substance under study..

1. Acceptance of urine sample – minimum 10 mL, in a sterile container

2. Sample preparation (extraction, hydrolysis, purification)

3. LC-HR-MS/MS analysis containing multi-level confirmation of the presence of test substances

4. Issue a report with interpretation of the results (optional: compliance with WADA or pharmacological standards)

Turnaround time and report format

• Standard time: 5-7 working days

• Express mode: 48-72 hours (upon request)

• Format of the results: a PDF report including:

  • list of detected substances with data confirming the detection

  • expert interpretation (upon request)

Applications of anabolic steroid analysis

• Anti-doping control in professional and amateur sports

• Endocrine and pharmacological diagnostics

• Supervision of testosterone therapy (TRT)

• Clinical and pharmacokinetic studies

• Forensic and toxicological expertise

Why should you trust us?

• Many years of experience in LC-MS/MS analysis

• Modern apparatus

• Full data confidentiality and secure sample circulation

• Ability to analyze a single sample or cyclic monitoring

• Serving individual and institutional clients (laboratories, clinics, sports organizations)

The list of substances includes: